NeuropixelsV2e Headstage Neuropixels 2.0 Probe(s)

Note

The NeuropixelsV2eBeta Headstage functions nearly identically to the NeuropixelsV2e Headstage. Simply replace NeuropixelsV2eData with NeuropixelsV2eBetaData and set its DeviceName property to "HeadstageNeuropixelsV2eBeta/NeuropixelsV2eBeta".

The following excerpt from the NeuropixelsV2e Headstage example workflow demonstrates Neuropixels 2.0 probe functionality by streaming data and saves Neuropixels 2.0 probe data. The second chain is disabled by default, assuming that only one probe is connected to the headstage. If two probes are connected, the second NeuropixelsV2eData chain can be enabled to stream data from both probes simultaneously. To enable, select all nodes in the disabled chain and press Ctrl + Shift + D, or click Enable right-clicking the selected nodes.

The NeuropixelsV2eData operator generates a sequence of NeuropixelsV2eDataFrames using the following properties settings:

• BufferSize is set to 30. Therefore, each frame will contain a [1 x 30 sample] Clock vector and a [384 channel x 30 sample] AmplifierData matrix. The Neuropixels 2.0 probe samples at 30 kHz per channel so this corresponds to 1 ms of data.
• DeviceName is set to "HeadstageNeuropixelsV2e/NeuropixelsV2e". This links the NeuropixelsV2eData operator to the corresponding configuration operator.
• ProbeIndex is set to "ProbeA". This links the data generated by this probe to the probe in port A of the headstage.
• OrderByDepth is set to False. When it is set to False, channels are ordered based on their channel number (i.e., from 0 to 383). If it is set to True, then channels will be ordered by their electrode depth. Channels are ordered first by their Y-value, and if two electrodes share a Y-value then they are ordered by their X-value. This means that Channel 0 is now the bottom-left most electrode, regardless of what its normal channel number is. For a visualization of the electrode depth, check out the GUI page to see how the current electrode configuration is set up.

The relevant members are selected from the NeuropixelsV2eDataFrame by right-clicking the NeuropixelsV2eData operator and choosing the following Output members: Clock, and AmplifierData. The MatrixWriter operators save the selected members to files with the following format: np2-a-clock_<filecount>.raw and np2-a-amp<filecount>.raw, respectively.