What is ONIX?

ONIX is a data acquisition system for neuroscience, composed of various pieces of hardware. ONIX differs from other acquisition systems in three major points:

1. Standards

All acquisition systems follow specific sets of rules that outline how data is structured and transmitted between parts of the system. For instance, Intan headstages and the classic Open Ephys Board use a Serial Peripheral Interface (SPI) for communication. Many different and non-interchangeable interfaces and protocols are in use, which is why each type of probe or camera usually comes with its own specific acquisition board.

The Open Neuro Interface (ONI) is an open standard that was designed as a unified protocol to communicate with a wide variety of instruments. By specifying how data should be organised but remaining agnostic to the type of data, a single acquisition system can be used to acquire from, for instance, extracellular probes, cameras, and tracking systems.

This means that the same software and hardware can now be used for a wide range of different recording instruments, so that labs do not have to spend time and money purchasing separate acquisition systems for each extra tool they wish to add to their experiment. Additionally, for those who want to develop new tools to study the brain, ONIX provides a powerful hardware backend and software infrastructure that can be reused to control almost any type of recoding instrument.

2. Tethers

There is a growing appreciation of experiments that examine the natural behaviours of animals. This often means using larger and more intricate (perhaps 3D) experimental setups. It also means that the animal should be impaired as little as possible by the recording setup. To achieve this it is of course important to reducing the weight of the headstage, but the weight of the tether that connects the headstage to the acquisition system is often overlooked. As the animal explores the arena, the centre of mass of the tether is rarely directly above the animal. Instead, it is off to one side, introducing a rotational force on the animal. The animal must compensate for this torque in order to keep its head up straight. Because the ONI specification allows communication over a single wire, ONIX uses a single coaxial cable, making ONIX tethers lighter and thinner compared to classic acquisition systems. ONIX tethers are 0.1 to 0.4 mm in diameter and are extremely flexible.

The tether must also be able to rotate as the animal does to prevent it from becoming twisted. Commutators are hardware devices that allow the tether to rotate while maintaining an electrical connection to the rest of the path to the acquisition system. As the animal moves through the arena, the ONIX commutator receives 3D tracking information from the headstage and drives a motor to actively rotate the tether, preventing twisting.

3. Latencies

In closed-loop experiments, data is not only acquired, but also processed and acted upon. For instance, one can provide optogenetic stimulation to a brain area every time a certain type of event is detected by an extracellular probe. The closed-loop latency of the acquisition system describes how much time passes between the initial event and the response of the system. In classic acquisition systems, this time is primarily spent on transmitting and processing acquired data, which becomes more and more challenging as the number of channels on a probe increases. A short latency allows the user to respond on the timescale of the biological event; for instance, within the integration window of a neuron.

Many classic acquisition systems rely on a USB connection between the acquisition board and PC. The slower transfer characteristics of USB means that a typical closed-loop latency would be in the range of several to tens of milliseconds. This is a considerable duration for the brain, as it is notably longer than the average action potential duration of around 1 ms. ONIX has much shorter latencies, of around 150 microseconds, because the host board of ONIX is directly connected to the acquisition PC, in a PCIe slot. This means that the system can respond quickly to detected events. It also means that time is freed up for this detection itself; by reducing the overhead time, more complex analysis can be run to extract the phenomenon you are interested in.