Differences Between Generations#

The Acquisition Board design has evolved over the years to add features and improvements.

Different combinations of the Acquisition Board main board and the FPGA module make up different Acquisition Board generations.

Although newer generations are backwards compatible and all generations generally work in the same way, there are some important distinctions to consider. This page contains about information about these distinctions.

Identify Your Acquisition Board#

../_images/acq_board_back_gen3.png

Gen 3 Acquisition Boards have a single power supply input on the Open Ephys FT600 USB board FPGA module and a Harp Clk Out port. The case has the 3d logo on the top.

../_images/acq_board_back_gen2.png

Gen 2 Acquisition Boards have a single power supply input on the Open Ephys FT600 USB board FPGA module.

../_images/acq_board_back_gen1.png

Gen 1 Acquisition Boards have two power supply inputs, one on the Opal Kelly XEM6310-LX45 FPGA module and one on the main board.

Hardware Differences#

This table can help you understand the hardware differences that define each generation.

Acquisition Board Generation

Gen 3.
Get 3D data
Gen 2.
OE FPGA module
Gen 1.
More channels
Gen 0.
The original

Release Date

May 2025

Dec 2022

Main version produced by OEPS from 2016 until Dec 2022.

Original design from 2010. Some boards shipped in 2022 due to shortage

Case

Open Ephys and 3d logos

Open Ephys logo

Open Ephys logo

Open Ephys logo

Main Board Version

v3

v2.3-2.4

v2.3

Up to v2.2

FPGA Module

Open Ephys
FT600 USB board
Open Ephys
FT600 USB board
Opal Kelly
XEM6310-LX45
Opal Kelly
XEM6310-LX45

USB Connection

USB3.0

USB3.0

USB3.0

USB2.0

Power Supply

Only one on the FPGA module.

Only one on the FPGA module.

One on the main board and one on the FPGA module.

One on the main board and one on the FPGA module.

Drivers, Gateware and Software Compatibility#

Different generations may require different drivers, gateware and software versions to operate, as per the table below.

Acquisition Board Generation

Gen 3.
Get 3D data
Gen 2.
OE FPGA module
Gen 1.
More channels
Gen 0.
The original

Drivers

FTD3XXDriver

FTD3XXDriver

FrontPanelUSB

FrontPanelUSB

Latest Gateware

Stored onboard
Stored onboard
Distributed with the Open Ephys GUI
Distributed with the Open Ephys GUI

Open Ephys GUI Compatibility

Open Ephys GUI 1.0 and above using Acquisition Board plugin

Backwards compatible with GUI version 0.6.x using OE Acquisition Board plugin (except no 3D capabilities)

Open Ephys GUI 1.0 and above using Acquisition Board plugin

Open Ephys 0.6.x using OE Acquisition Board plugin

All Open Ephys GUI versions using Acquisition Board plugin

All Open Ephys GUI versions using Acquisition Board plugin

Bonsai Compatibility

Not supported yet

Bonsai.OpenEphys with pending improvements

Bonsai.Ephys

Bonsai.Ephys

Functionality Differences#

This table is meant to guide you in regards to functionalities across generations and differences you might notice during operation.

Acquisition Board Generation

Gen 3.
Get 3D data
Gen 2.
OE FPGA module
Gen 1.
More channels
Gen 0.
The original

Channel Count

512

512

512

256

Streams Orientation Data

Yes, using headstages with 3D capabilities

No 3D capabilities

No 3D capabilities

No 3D capabilities

Compatible with Open Ephys SPI commutator

Yes

No 3D capabilities

No 3D capabilities

No 3D capabilities

Analog Inputs

±5V

±5V or 0–5V

±5V or 0–5V

±5V or 0–5V

Harp Enabled

Yes, integrated using dedicated Harp Clk Out port

Yes, through Harp timestamp generator (for Acquisition Board) on any HDMI port

Not Harp enabled

Not Harp enabled

Power Light

Red

Red

Green

Green