Miniscope v4.5#

image of ucla miniscope rotating and example data

UCLA Miniscope v4 Features & Properties#

Mechanical Properties:

  • Mass: <3g

  • Dimensions: 14.5 × 18 × 22.5mm

Optical Properties:

  • FoV: 1 × 1mm

  • WD: 675 ± 250µm

    • The dimension following the ± symbol refers to the WD’s dynamic range from the UCLA Miniscope v4’s electrotunable lens

  • The above specifications can be adjusted by reconfiguring the objective module

  • GRIN Lens Compatibility: relay GRIN lenses (i.e. \(pitch = 0.5n, n=1, 2, 3...\))

  • Spectral Compatibility:

    • Common green fluorophores such as GCaMP

    • Common red fluorophores such as jRGECO

Sensor (Python480) Features:

  • Adjustable Frame Rate: 10, 15, 20, 25, or 30 fps

  • Adjustable Exposure: three discrete levels (low, medium, and high)

  • Bit Depth: 10

  • Sensor Pixel Dimensions: 608 pixels × 608 pixels

Software Features:

  • GUI Compatibility: Bonsai GUI (recommended) or UCLA-Miniscope-DAQ-Software (deprecated)

  • Sensor settings and imaging depth adjustable through software

  • Real-time visualization and logging of orientation and image data

Additional Features:

  • On-board orientation sensor (BNO055)