Workflow Tutorial

Note

An Open Ephys Coaxial Commutator is necessary for this workflow

1. Download the following workflow (.bonsai file) and open it with Bonsai:

   

   

   uclaminiscopev4-miniscopedaq-commutate.bonsai

   

2. Set the UCLAMiniscopeV4 operator’s Index property to the value that corresponds to the index of your miniscope.

   

   1. Set the Index value to 0 by editing the Index field that appears in the Properties pane after left-clicking the UCLAMiniscopeV4 node.

   2. Test the selected Index value by starting the workflow [1] and double left-clicking the Image node. If the displayed video stream corresponds to that of your miniscope, proceed to the next step. Otherwise, increment the Index value by 1 and repeat this step.

   Note

   A device index specifies a camera device. If there are additional cameras connected to your PC (including laptop webcams), finding the correct index value might involve an iterative trial-and-error process.

3. Set the COM port associated with your commutator in the workflow

   • Left-click the Commutator node and edit the PortName property under the Properties pane to match the port that corresponds to your commutator.

   Note

   If you are uncertain about which COM port corresponds to your , follow these instructions:

   1. Open Window’s Device Manager.

   2. Unplug the commutator, and plug it back in. Observe which COM port disappears and appears in device manager when doing so - that is the COM port associated with your commutator. If the commutator does not appear in device manager, follow these instructions

[1]

Start a workflow by left-clicking the Start button (indicated by green triangle) at the top of the Bonsai workflow editor or pressing F5 while the Bonsai workflow editor is the active window.